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KMID : 0903619870280010088
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Journal of the Korean Society for Horticultural Science
1987 Volume.28 No. 1 p.88 ~ p.98
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Effect of Explant Source , Breaking of Apical Dominance and Dormancy on Shoot Proliferation and Bulbing of Hyacinth , Narcissus and Lily in vitro
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Abstract
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Tissues of scales, inflorescence stem and foliage leaves of hyacinth, narcissus and lily were cultured on Murashige-Skoog medium for their micropropagation, and the effect of growth regulators on the bulblet and shoot formation, and shoot removing on the breaking of dormancy as well as apical dominance were studied in this experiment.
In case of hyacinth, the rate of bulblet and shoot formation was different depending on the cultivars and tissues cultured. However, no differences on organogenesis were observed both in 1.0 ㎎/ℓ of IAA and IBA tested. Inflorescence stem and foliage tissues produced more bulblets and shoots than scale tissue segments except cv. Junbos. l~iultiple shoots were induced by reculturing regenerated shoots with basal parts producing more multiple shoots than distal.
The breaking of dormancy was achieved by 2∼4 weeks chilling treatment of bulblets. Removal of shoots after dividing bulblets produced more multiple shoots than simple removal of shoots from distal part of bulblet did.
In narcissus, the rate of bulblet formation was different depending on the cultivars used. The rate of shoot formation was greater by using twin scale segments than by inflorescence stem. Chilling treatment of 4 weeks promoted shoot formation by breaking dormancy. The removal of shoot formed resulted in breaking of apical dominance. The removal of shoots after splitting bulblets was effective in the formation of bulblets and multiple shoots.
In lily, IBA treatment was more effective in increasing bulblet weight than kinetin. But the latter promoted leaf formation, and the number of scales was increased by removing leaves formed.
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